will be the last guest on Ellen DeGeneres' talk show, it was announced on Tuesday.
The final episode of the long-running will air on May 26 with Pink and as guests as well.
Having the A-list star visit the show on Ellen's very last day serves as a bookend of sorts as 19 years ago the Friends veteran was the comedienne's very first guest.
This will be Jennifer's 20th time as a guest on Ellen.
Last call: Jennifer Aniston will be the last guest on Ellen DeGeneres' talk show it was announced on Tuesday. The final episode of the long-running daytime series will air on May 26
Other guests that will appear before the finale include Oprah Winfrey, Julia Louis-Dreyfus, Mila Kunis, Kate McKinnon, Justin Timberlake, Keith Urban, Zac Efron, Sean 'Diddy' Combs, Luke Bryan, Kerry Washington, Brad Paisley and Bruno Mars.
In September Aniston appeared on the debut of the last season as she wore all black.
But she teared up as soon as she walked out on stage, realizing the show really is coming to an end.
Ellen announced in May of 2021 the show was ending following complaints of a toxic workplace from staff on the show that led to an internal probe from Warner Bros.
Sad to say bye: In September Aniston appeared on the debut of the last season as she wore all black. But she teared up as soon as she walked out on stage, realizing the show really is coming to an end
Jennifer became teary-eyed when Ellen said the Hollywood star has been a part of the show since 'day one' and introducing her as a 'friend.'
As soon as Jennifer walked out onto the stage, she started crying and warmly embraced Ellen. Jennifer threw kisses at the audience before wiping away her tears with a tissue.
Wearing a one-shoulder top, Jen said: 'What the hell? This is not supposed to be emotional, God dang it! Not yet - it's not even over, you know I have not been out in a long time, like two years, and I am not kidding, and the last time I was here hosting a show was here when you were at the DMV'.
Emotional: Jennifer put her hand to her face as she could not control her emotions
Weepy: As soon as Jennifer walked out onto the stage, she started crying and warmly embraced Ellen. Jennifer threw kisses at the audience before wiping away her tears with a tissue
Tears: Ellen said that Jennifer has been a part of the show since 'day one'
Ellen, in a green suit with a floral shirt, said thanks to Jennifer as she knows she does not go out a lot and is being careful during COVID-19.
The Finding Dory star will bring her long-running eponymous daytime show to an end in the coming months and the star-studded line-up for season 19 - which will have a fully-vaccinated studio audience - is headed by the former Friends star, who was her pal's first ever guest when the program began in 2003.
The first episode will also see make his 20th appearance on the show, while other guests in the first week will include - who will give her first sit-down interview since Keeping Up With the Kardashians came to an end - Imagine Dragons and Tiffany Haddish.
Soooo 2003! Having the A-list star visit the show on Ellen's very last day serves as a bookend of sorts as 19 years ago the Friends veteran was the comedienne's very first guest. Aniston looked like she just stepped off the beach in Malibu
And that's not all as later in the season Melissa McCarthy, Julianne Moore, Melissa Etheridge and Sean 'Diddy' Combs will be among the stars to take their seats on Ellen's famous sofa for the last time.
Season 19 of The Ellen DeGeneres Show began on Monday September 13.
Ellen announced in May she would bring the show to an end in 2022.
In a pre-recorded video from her show, shared on her Twitter account, she told her virtual audience: 'I am announcing that next season, season 19, is going to be my last season.
First time for everything: At the time Jennifer was married to Brad Pitt and wore her wedding band
Ellen - who is married to Portia de Rossi - pondered the decision 'for a while', but also admitted that when she penned a three-season extension two years ago she knew then the 19th series would be her last.
She added: 'I've thought a lot about this decision, I sat with it for a while. I meditated on it, I talked to Portia, I talked to myself.
'Two years ago, I signed a deal for three more years, and I always knew in my heart that season 19 would be my last.
Regular fixture: Here Jennifer is seen in a previous season of Ellen
That kiss! They locked lips on one of the shows on the Burbank, California set
'Nineteen is a great number. The 19th amendment gave women the right to vote, and I believe women should be allowed to vote.
'You may wonder why I've decided to end after 19 seasons and the truth is I always trust my instincts. My instinct told me it's time. I truly have felt like next season was the right time to end this amazing chapter.
'I promise you that we are going to have a fantastic final season. It will be a season where I truly get to say, thank you. Every day will be a celebration.'